Search results for " harvest"
Article
Challenges in Cell Harvesting Prompt Enhancements
Cell harvesting involves the separation of the cell-culture from the medium and running it through several techniques: centrifugation, microfiltration, depth filtration, and filtration through absolut…
Article
Automated harvesting and 2-step purification of unclarified mammalian cell-culture broths containing antibodies
We report on a new integrated platform for automated harvesting of whole unclarified cell-culture broths, followed by in-line tandem affinity-capture, pH neutralization and size-exclusion chromatograp…
Article
Downstream Processing for Cell-Based Therapies
…emphasis on large-scale cell expansion; however, equally important is the development of large-scale harvesting and downstream processing operations. This paper presents and summarizes some of the ch…
Article
Combining Microbioreactors and Advanced Statistical Techniques to Optimize a Platform Process for a New Host-Cell Line
Figure 2: Characterization of titer at harvest for a model two-dimensional process space spanning a range of dissolved oxygen tension (DOT) and pH values.
By using DoE, it is po…
Article
Adherent Cell Culture in Biopharmaceutical Applications: The Cell-Detachment Challenge
While cell attachment may prove advantageous for some purposes, such as supernatant harvesting, cell detachment may become an enormous challenge when cells are to be harvested. Cell detachment is prob…
Article
Overview of a scale-up of a cell-based influenza virus production process using ReadyToProcess equipment
As shown in Figure 4, the infectious and total amount of virus particles initially increased quickly and then remained at a constant level until time of harvest, when the amount of virus particles was…
Article
Single-Use Technologies Prove Effective for Viral Vector Process Development
The presence of serum, culture harvest viability, turbidity, cell lysis reagents, contaminant DNA levels, and residual transfection agents are all upstream factors. Overall process scalability and rob…
Article
A Risk-Based Genetic Characterization Strategy for Recombinant CHO Cell Lines Used for Clinical and Commercial Applications
Finally, the presented strategy includes the late-phase characterization of the expression construct using cells at the limit for in vitro cell age harvested from the commercial cell-culture process t…
Article
Technology Innovations Improve Process Chromatography Performance
“It doesn’t help that there’s approximately 30% more effort, such as resources, materials, and equipment, as harvest material goes through downstream purification due to multiple unit operations. Any …
Article
Gene Therapies Push Viral Vector Production
Gene Therapies Push Viral Vector Production
Viral vectors show promise as a delivery mechanism for gene therapy, but which virus types are commercially viable?
By Feliza Miraso…